Epitope Mapping Walnut Allergens Jug r 1 and Jug r 2

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Linear IgE epitope mapping of the English walnut (Juglans regia) major food allergen, Jug r 1.

BACKGROUND Peanut and tree nut allergies can be life-threatening, and they appear to be growing in prevalence. Jug r 1, a 2S albumin seed storage protein, was previously characterized as a major English walnut food allergen. OBJECTIVE We sought to identify the linear IgE-binding epitopes of Jug r 1 and to determine which, if any, amino acids are necessary for this binding to occur. METHODS ...

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Jug r 2-reactive CD4+ T-cells have a dominant immune role in walnut allergy

BACKGROUND Allergic reactions to walnut can be life-threatening. Although IgE epitopes of walnut have been studied, CD4(+) T cell-specific epitopes for walnut remain uncharacterized. In particular, the relationship of both phenotype and frequency of walnut-specific T cells to the disease have not been examined. OBJECTIVES We sought to provide a thorough phenotypic analysis for walnut-reactive...

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Microarrayed Jug r 1 shows the best diagnostic performance in walnut allergy

Several walnut (WN) allergens have been identified to date: Jug r 1 (2S albumin), Jug r 2 (7S globulin), Jug r 3 (LTP), Jug r 4 (11S globulin) and Jug r 5 (profilin), but their clinical significance is still to be determined in some cases. Allergen microarray (ISAC) provides information for three of these allergens. The aim of the study was to determine the importance of component resolved diag...

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Jug measuring: Algorithms and complexity

We study the hardness of the optimal jug measuring problem. By proving tight lower and upper bounds on the minimum number of measuring steps required, we reduce an inapproximable NP-hard problem (i.e., the shortest GCD multiplier problem [G. Havas, J.-P. Seifert, The Complexity of the Extended GCD Problem, in: LNCS, vol. 1672, Springer, 1999]) to it. It follows that the optimal jug measuring pr...

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Identification and implication of an allergenic PR-10 protein from walnut in birch pollen associated walnut allergy.

SCOPE English walnut (Juglans regia) belongs to the most important allergenic tree nuts. Co-sensitization with birch (Betula verrucosa) pollen has been reported. We aimed to identify a walnut allergen homologous to the major birch pollen allergen Bet v 1. METHODS AND RESULTS A cDNA encoding a Bet v 1-homologous allergen (Jug r 5) in walnut kernels was cloned by RT-PCR. Jug r 5 was expressed i...

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ژورنال

عنوان ژورنال: Journal of Allergy and Clinical Immunology

سال: 2018

ISSN: 0091-6749

DOI: 10.1016/j.jaci.2017.12.570